Identification and Characterization of Novel SPHINX/BMMF-like DNA Sequences Isolated from Non-Bovine Foods

Sixteen novel circular rep-encoding DNA sequences with high sequence homologies to previously described SPHINX and BMMF were isolated for the first time from non-bovine foods (pork, wild boar, chicken meat, Alaska pollock, pangasius, black tiger shrimp, apple, carrot, sprouts alfalfa, radish, broccoli). The phylogenetic analysis of full-length genomes grouped these together representatives SPHINX/BMMF group 1 2 (eight in each group). characterization genome lengths, genes present, conserved structures confirmed their relationship known sequences. Further iteron-like tandem repeats 1-related revealed a correlation both tree branches as well Rep protein was able differentiate subtypes members. For members, distinct grouping into two clades (A B) subgroups could be detected. A deeper investigation potential functional regions upstream rep gene new dso sso plasmid groups that replicate via rolling circle mechanism. Phylogenetic analyses accomplished by different ssDNA viruses, pCRESS, plasmids replication mechanism, this yielded insights proteins. clear relation proteins confirmed. Interestingly, also verified. Furthermore, theta-replicating Reps is discussed.